Tubulins are a major component of ciliary microtubules and the principal protein subunit of microtubules in the cell cytoplasm.
(73) Second, axonemal
tubulins undergo several types of post-translational modifications, including acetylation of [alpha]-tubulin and polyglutamylation and polyglycylation of both [alpha]- and [beta]-tubulin.
They show how CaMKII kinase domains can collectively bind and phosphorylate 6-bit bytes, resulting in hexagonally-based patterns of phosphorylated
tubulins in microtubules (Figure).
For example, many protist and fungal
tubulins are only slightly affected by taxol (5).
Both basic and clinical sciences are investigating the family of proteins called the
tubulins, which form microtubules that are proving important in both disease mechanisms and therapies.
Tubulins are the proteins that form microtubules, which are key components of the cellular cytoskeleton (structural network).
All preparations were washed in phosphate buffered saline, (PBS, pH 7.2), then incubated in PBS containing mouse monoclonal anti-[alpha] and anti-[beta]
tubulins (Sigma T9026, T-4026, 1:1 mass mixture) pre-bound with Zenon[TM] Alexa Fluor 488 [F.sub.ab] (Z-25002, 1:1 by mass; Molecular Probes).
"If they have truly identified the origin of eukaryotic
tubulins, then it would be extremely important," says Jeffery Errington of Oxford University in England, who studies a bacterial protein with actinlike properties (SN: 3/31/01, p.
All have been found in the parasite's cytoplasm often as complex proteins, i.e., ribosomes, nucleosomes (histones), chaperonines, structural proteins (
tubulins, kinesin), glycosomes (triose phosphate isomerase).
Microtubules were labeled with mouse monoclonal anti-[alpha]
tubulin (Sigma-T9026) diluted to 0.5 [micro]L/mL by 1% BSA/PBS (containing 0.1% TritonX) by incubating for 24 h at room temperature.
It binds to AY-
tubulin and thus preventing polymerization of
tubulin dimers to microtubules (Lacey, 1988).