Busch and Russell (1964) studied 31
sublines from two inbred lines (Os420 and M14) and reported biological changes in more than one character in a
subline.
The resistant SW620 Ad300
subline was developed from its parental cell line SW620 by stepwise selection in increasing concentrations of doxorubicin.
On MTT toxicity assay, the established MG-63
subline (Fig.
They included drug-sensitive CCRF-CEM and multidrug-resistant P-glycoprotein over-expressing CEM/ADR5000 leukemia (Efferth et al., 2003b; Gillet et al., 2004; Kimmig et al., 1990), the MDA-MB-231-pcDNA3 breast cancer and its resistant
subline MDA-MB-231 -BCRP clone 23) (Doyle et al., 1998), the HCT116 ([p53.sup.+/+]) colon cancer cells and its knockout clones HCT116 ([p53.sup.-/-]), the U87MG glioblastoma cells and its resistant
subline U87MG.
FIW 180, a wild-type fibroblast cell line, was established at the Cancer Center in Warsaw (Poland), MRCS, a fetal fibroblast cell line, was purchased from Flow Co., and a human colon cancer cell line that is a
subline of DETA was a gift from the Cancer Institute in Vienna.
SW620 and its drug selected P-gp overexpressing SW620 Ad300
subline were kindly provided by Dr.
K562/VCR, a vincristine (VCR)-resistant
subline of K562 cells that overexpresses MDR1 (17), was generously provided by Dr.
In this study, results showed that the cell viability of A549/D16
subline is decreased when treated with psoralen plus DOC, while psoralen has no effect on the cell proliferation on A549 and A549/D16 cells.