Disease was recorded using 0-4 infection scale [14]: (0) no
uredia or other macroscopic sign of infection, (;) no
uredia, but hypersensitive necrotic or chlorotic flecks present, (1) small
uredia surrounded by necrosis, (2) small to medium
uredia often surrounded by chlorosis; green islands may be surrounded by chlorotic or necrotic border, (x) random distribution of variable-sized
uredia on single leaf, (3) medium sized
uredia that may be associated with chlorosis, and (4) large
uredia without chlorosis.
Uredia stripes formed on the leaves at mature stage of the planted give yellow and dusty appearance.
Each race was originally purified by single spore isolation or by transfer of single
uredia for several generations.
The infection type was recorded as 6 classes (0 to 4) according to the methods also described by Luo et al (2005), respectively as follows: 0, no visible symptoms; 0;, few necrotic flecks; 1, small sporulating
uredia surrounded by necrotic tissue; 2, small to medium
uredia with chlorosis and necrosis; 3, moderately sized sporulating
uredia surrounded only by chlorotic tissue; and 4, abundantly sporulating
uredia without chlorosis.
([paragraph]) Rust reaction; 1 = immune, no pustules; 2 = small pustules; 3 = intermediate pustules
uredia less than 0.3 mm diameter, 4 =
uredia 0.3 to 0.5 mm diameter, 5 =
uredia 0.5 of 0.8 mm, and 6 =
uredia larger than 0.8 mm diameter (Stavely et al., 1983).
S###Susceptible###Large
uredia are present, generally with little or no chlorosis or necrosis.
pachyrhizi's
uredia in soybean leaflets treated with the EO of CI.
IT designations of plants refer to: 4 = extremely susceptible with large
uredia; 33- = moderately susceptible with combination of medium size (3) and small size (3-)
uredia; 0; = resistant with necrotic flecks; and 0;N = resistant with necrotic lesions.
Plants of the HA-R6, HA-R7, and HA-R8 germplasm populations had an immune reaction (no
uredia) to race 777.
The spreader rows were removed when the first
uredia were observed on the known susceptible cultivars in the trial (Table 2).