arrayer

Ninety prostate cancer specimens were used to construct a tissue microarray by manual tissue arrayer. Representative areas of each paraffin block were labelled by B.K.

Urinary proteins were spotted on the surface of an epoxy modified glass slide using a Smart Arrayer 48 (Capital Bio Corp.

El tissue arrayer consta de dos agujas de distintos calibre con las que se realiza el troqueleado de los bloques.

(13) A Beecher Instruments Manual Tissue Arrayer (Sun Prairie, Wisconsin) was used to prepare tissue cores from selected regions of archival tissue blocks.

Tissue microarray (TMA) of 1.0 mm core size was constructed as described previously [32] using a semiautomatic Tissue Arrayer Minicore (Alphelys, SAS, France).

Each capture antibody diluted to 100 [micro]g/mL in PBS-Trehalose (Dulbeccos's phosphate buffered saline containing 50 mM D-(+) Trehalose) was loaded onto a 384-well plate (Genetix) and printed in in four sets of triplicate spots in a 16 x 16 array format onto a poly-l-lysine-coated glass slide (Thermofisher, UK) using a Biorobotics Microgrid II arrayer (Microgrid) and a silicon contact pin (Parallel Synthesis Technologies, USA).

From each carcinoma, two tissue cores (1 mm in diameter) were obtained from the main tumor mass and two cores were taken from the tumor at the positive surgical margin; these samples were arrayed in a recipient paraffin block using MTA Booster OI Manual Tissue Arrayer (Alphalyse, Plaisir, France).

Pure tumor areas from the primary Gleason grade were punched with use of the manual tissue arrayer (Beecher Instruments Tissue Microarray Technologies), creating cores 0.6 mm in diameter and 5 mm in length.